I have been told that confocal-like images with optical sectioning
capability can be obtained without using laser confocal microscope hardware.
One obtains images using a standard microscope and ccd camera as one steps
along in z (ie numerous changes in focus). These blurred images are
processed together along with the point spread function for the optical
system to produce in-focus images at the numerous z-planes. Can someone
tell me:
1. Where I can find a good description of this approach.
2. Where I can obtain (free? buy?) the deconvolution algorithm.
3. If there is an accepted name for this approach.
4. What are the tradeoffs between the hardware and software approaches
in x, y and z resolution and acquisition time.
Thank you in advance for any asssistance
Lauren M. Peterson (313) 994-1200 Ext 2284
ERIM (313) 994-5704 FAX
Letter Address: P.O.Box 134001
Ann Arbor, MI 48113-4001
Package Address: 1975 Green Rd.
Ann Arbor, MI 48105-2554
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