Linda, ive used a vibratome to try to freshly cut mammary gland tissue. I
also had a hard time. You could try to surround your tissue with a high
percent agarose containg block.. thats supose to help. also you can glue
(elmers) the tissue to the platform to hold it in place, just dont use
that part of the tissue for further studies. Good luck.
What i eventually used was a tissue chopper. and if your careful with it,
you shouldnt get to much autofluorescence.
good luck
Julie

On Thu, 27 Feb 1997, Linda Graeter wrote:

> Hello all,
>
> We have a study coming up that will require thick vibratomed sections of
> fresh skin (rodent and human). Does anyone have experience in this?
> We have tried mounting the skin on several types of materials but are still
> having problems.
>
> Thanks to anyone that has time to reply,
>
> Linda J. Graeter, Ph.D.
> ManTech Environmental
> Toxicology Division
> WPAFB, OH
> [log in to unmask]
>