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February 1997

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Subject:
Re: Temperature influence on focal depth
From:
"Steve Gaffin, Ph.D." <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 10 Feb 1997 15:36:14 EST
Content-Type:
text/plain
Parts/Attachments:
text/plain (435 lines) 
It's more than focal depth that changes.  Intracellular ion concentrations may
also change.  See attachment.  Good luck.

______________________________ Reply Separator _________________________________
Subject: Temperature influence on focal depth
Author:  Confocal Microscopy List <[log in to unmask]> at
Internet-Mail
Date:    2/10/97 3:31 PM


THE PROBLEM:
We study living cells and have some problems with temperature
controlling. It seems that a variation of less than 0.1 degree C influences the
focal depth too much. The focal depth changes sometimes 0.5 um.

THE SET-UP:
  1) 60x/1.4 Nikon (on a Biorad MRC 1000/1024)
  2) an objective heater and its controller.
  3) cells on a Glass Bottom Petri dish (MatTek)
  4) a 'warm table' for the petri-dish and its controller

The heater controllers keep the temperature on 37 C with an accuracy
of less that 0.1 C so, that seems to be good enough.

THE QUESTION:
  1) Has anyone similar problems?
  2) Did anyone solve the problem?


Thank for your reply, Erik Manders

CRC Nuclear Structure and Function Group
Sir William Dunn School of Pathology
Oxford University
South Parks Road, Oxford OX1 3RE, UK
Tel: (+44/0) 1865 275529 (/275500)
Fax: (+44/0) 1865 275501
E-Mail: [log in to unmask]

The following is an attached File item from cc:Mail.  It contains
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end

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