We are using Hypaque as a mounting/clearing agent for our DiI/DiO
preps and works as well as anything we have tried. The vibratome
sections need to be viewed and data sets collected on the confocal within
2-3 hours of being cut. We have collected them as late as 12 hours after
cutting and mounting but they have started to bleed by then. If there is a
better clearing agent for the lipid soluble dyes, I would be interested.


Patty Jansma                                    Tel:520-621-6671
[log in to unmask]
Arizona Research Labs Division of Neurobiology
University of Arizona

On Mon, 10 Mar 1997, Zen Faulkes! wrote:

> Judy Drazba wrote:
> >
> > Could someone recommend an anti-fade mountant for use with DiI/DiO labelled
> > cells.  The usual glycerol-based mounting media cannot be used because the
> > dyes "bleed." Molecular Probes recommended something called Cytoseal.  Has
> > anyone tried it?  Any other suggestions?
> >
> > TIA
> > Judy
> >
>
> I have also had this problem of "bleeding" with DiI and DiO and is
> currently looking for a way to mount and clear stained preps.  I have
> also used glycerol as well a radiopaque contrast medium called Hypaque
> at someone's reccomendation, but also encountered the problem of
> bleeding.  I would also appreciate any suggestions on this matter.
>
> Thank you
>
> Francine Lewis ([log in to unmask])
> Dept. of Biology,
> McGill University
> 1205 Avenue Dr. Penfield
> Montreal PQ H3A 1B1
> Canada
>