I'm confused, why would an '...intensity of 50 be 2,5, (or even 20
fold less) (in terms of amount of antigen) than an intensity of
150...'? 50 is 3 fold less than 150 (assuming no background). The
other problem is that any bleaching will make such comparisons
impossible. Other than that the A/D converter should be completely
linear within it's 8 bit range..

Regards Mark Cannell



On Mon, 18 Aug 1997 13:57:50 -0400 Wayne Arnold wrote:

> From: Wayne Arnold <[log in to unmask]>
> Date: Mon, 18 Aug 1997 13:57:50 -0400
> Subject: intensity vs amount of protein
> To: [log in to unmask]
>
> I am using a Biorad MRC 600.  Is there a way to correlate
> differen't intensities with amount of antigen present in an
> image. ie I don't want to quantify the antigen, I just need
> to know at a given setting for the photomultiplier can I
> determine if say an intensity of 50 is 2,5, or 20 fold less
> (in terms of amount of antigen) than an intensity of 150.
>
> Wayne