On Aug 25,  4:29pm, Beat Ludin wrote:
> Subject: Yokogawa CSU10 (Nipkow disk confocal)
>
> comparison to non-confocal video fluorescence microscopy:
> + illumination confined the the scanning plane, so there should be less
> phototoxicity problems.

I dont understand this -- the cone of illumination is similar to that of a
conventional laser scanning confocal. Perhaps someone on the list can explain
to me what I am missing.

> + elimination of problems with culture medium autofluorescence.

I also dont understand this. Why should a nipkow disk system eliminate
autofluorescence. Perhaps it is the use of visible light excitation that
eliminates the autofluorescence.

--aryeh

--
Aryeh Weiss                          | email: [log in to unmask]
Department of Electronics            | URL:   http://optics.jct.ac.il/~aryeh
Jerusalem College of Technology      | phone: 972-2-6751146
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