CONFOCALMICROSCOPY Archives

September 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Ted Inoue <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 18 Sep 1997 15:45:06 -0400
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I can comment on item 2.
This is a very common occurrence with high resolution output devices because the device can actually render the pixel as a small, resolved square. Lower resolution devices do not show this artifact as severely because they can't render details that fine.

In detail:
You have an image with approximately 1000 pixels horizontally. This is spread out across 200mm. 200mm/1000pixels = 1/5 mm/pixel. At this resolution, you will see each pixel as a square 0.2mm across. Typically, this resolution is just about acceptable. However, imagine what happens if you window in on a smaller portion of the image, as is common. Suppose you now have an image which is only 250 pixels across. Now, when printed, the pixels will be 4/5mm and will hence look very "chunky".

To avoid such problems, you can take the image in a program like PhotoShop which can zoom the image with interpolation. You then save the image as zoomed, and an image which previously had 250 pixels can have 1000 or more. The magnification is empty, and all you've done is smoothed the transitions between pixels so that they are less evident when printed at high resolution.

A second, more straightforward approach is to tell the printer that you're printing at a lower resolution. If the printer setup supports such an option, you may be able to tell it that you're printing an image at 75dpi(dots per inch) for example. The printer should then scale the image as appropriate and render the image in a more aesthetic manner.  In the example of a 250 pixel image, printed at 75dpi, the resultant image would be 250/75 = 85mm.



-----Original Message-----
2. The image is very pixelated, although it looks fine on the monitor.



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