Have you tried the Lyso-phosphatidyl choline method.
I use this routinely. It is easy quick and clean. Not tried paraffin sections
though. Frozen sections worked well. The method is described in the Molecular
Probes Bible. If you need more details let me know.

On 18-Nov-97 Richard Hotchkiss wrote:
>I am trying to stain actin using Oregon Green labeled with Phalloidin.
>Tired both cells and parafin embedded tissues and neither has worked.
>Tried 2 lots of fluorescent probes and still no fluorescence.  Not sure
>if the cells are permeabilized and that the phalloidin is getting in.
>Tried using heat and triton (0.1%) to permeabilize the cells.
>
>Recommendations, methods, or references appreciated.
>
>Kevin Tinsley

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Date: 18-Nov-97
Time: 17:35:58

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