CONFOCALMICROSCOPY Archives

December 1997

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Subject:
Re: label ?
From:
Anna Smallcombe <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 22 Dec 1997 09:44:21 -0800
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     Dear Jan - have you tried the Moecular Probes catalogue or Web site -
     they have a whole section on thiol labelling and modification.

     Best regards,


     Anna Smallcombe PhD Bio-Rad


______________________________ Reply Separator _________________________________
Subject: label ?
Author:  Confocal Microscopy List <[log in to unmask]>  at
Internet
Date:    16/12/97 10:26


I am looking for a fluorochrome that is an alternative for MBrB of MClB. I
want to label thiols in living cells with a fluorochrome that has to be
excited with visible light (preferably 488nm) and becomes fluorescent after
reacting with the free SH groups.

Other labels (CMFDA/CMEDA) are already fluorescent after reacting with cell
esterases and only "stick" to SH then.

Who can help? Ever heard of OPT in this respect? Thank you for all good
advice.

Jan W Rijstenbil
NIOO CEMO
PO Box 140
NL-4400 AC  Yerseke
Netherlands

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