On Wed, 3 Dec 1997, Guy Cox wrote:

> >Hi,
> >
> >Members of my lab want to perform living microscopy of yeast (pombe) cells
> >expressing GFP and BFP fusion proteins. We are interested in viewing the
> >chromatin and would like a fluorescent dye in another wavelength.
> >
> >
>
>
> I'd suggest toto or topro (Molecular Probes) - excited with 647nm
> line and well out of the way of GFP.  Some of our users do triple
> labelling with them with very good results.

Do you think that toto is not domageable for the living cells, better than
other DNA dyes like DAPI, or effects are very similar ? (this question is
independant from the first one about double labelling)

>
>                                                 Guy Cox
>
> Dr. Guy Cox,   |                    ooOOOOOOoo
> E.M. Unit, F09 |        #       oOOOO  |  |  OOOOo       #
> Univ of Sydney |       ###    OOO|  |  |  |  |  |OOO    ###
> NSW 2006,      |       ###  OOO  |  |  |  |  |  |  OOO  ###
> Australia      |       ### OO |  |  |  |  |  |  |  | OO ###
> Phone:         |      #####   |  |  |  |  |  |  |  |   #####
> +61 2 9351 3176| =====#####============================#####=====
> Fax:           |      #####                            #####
> +61 2 9351 7682|    ~~#####~~~~~~~~~~~~~~~~~~~~~~~~~~~~#####~~
>

---------------------------------------------------------------------------
Christine Juge                            e-mail: [log in to unmask]
local 2115                                 phone: (418) 656-2131 poste 6119
Pavillon C.E. Marchand                       fax: (418) 656-7493
Universite Laval, Ste Foy, QC
G1K-7P4 CANADA