Try Congo Red.  We use our FITC filters to image plant cell walls stained
with Congo Red.  In your case this would probably mean you will need an
alternate secondary antibody fluorophore.



>Dear Fellow Confocalists,
>
>        I am interested in visualising cellulose microfibrils and
>microtubules/microfilaments in wood-forming cells of trees in the confocal
>microscope.  I use FITC-labelled antibodies for cytoskeleton and would like
>to stain the cellulose with a fluorescent dye.  If I had access to a UV
>laser I would have no hesitation in using calcofluor/tinopal, but I don't.
>So, can anybody suggest a visible light-excited fluorochrome that will
>localise the cellulose and will permit imaging of both cellulose and
>cytoskeleton?  (If it helps we have a Zeiss 510 with 488, 568 and 633 nm
>lines.)
>
>        I thank you in advance,
>
>        Yours sincerely,
>
>        Nigel Chaffey
>
>-----------------------------------------------------
>Dr Nigel Chaffey,
>Dept Forest Genetics & Plant Physiology,
>Swedish University of Agricultural Sciences,
>S-901 83 Umeå,
>Sweden
>Phone: +46-90-786-6305
>Fax: +46-90-786-5901
>eMail: [log in to unmask]
>
>Looking for another job/position/post...


 R. Howard Berg, Ph.D.
 Department of Microbiology &
        Molecular Cell Sciences
Campus Box 526041
University of Memphis
Memphis, TN, 38152-6041
E mail: [log in to unmask]
phone: 901-678-4449   fax: 901-678-4457