Dear Renato - I have not personally used PE, but I know it has a very
     broad excitation spectrum. Also, it is quite a large molecule and may
     not be the best for antibody penetration to internal antigenic sites.

     Best regards

     Anna Smallcombe (PhD Senior Applications Specialist)


______________________________ Reply Separator _________________________________
Subject: Pycoerythrin
Author:  Confocal Microscopy List <[log in to unmask]>  at
Internet
Date:    23/04/98 16:58


Dear All
Has anyone out ther any experience with PE-conjugated antibodies in
confocal microscopy ? I'm trying to double label cells (using FITC
secondary antibodies) and monoclonal coupled to PE. As far as I
understand, PE absorbs at both 488 and 565, emitting at 578. So, in
principle one should be able to discriminate FITC and PE based on
their different emissions, which I can discrminate at the microscope
eyepiece. However, at the detector level (on a BioRad 1024) the
signal obtained using excitation at 488 or 568 and emission at 578
are quite the same.
If anyone has had success using PE-labeled antibodies in
double-labeling exepriments on confocal microscopy, please contact
me directly.
Thanks

.
Dr. Renato Arruda Mortara
Division of Parasitology
Universidade Federal de Sao Paulo
ESCOLA PAULISTA DE MEDICINA
Rua Botucatu, 862 6o. andar
04023-062 Sao Paulo SP
Brasil
Email [log in to unmask]
Fax : 55 11 571-1095
Fone: 55 11 570-8306