You might want to check "Fluorescent and Luminescent Probes for Biological
Activity" - edited by WT Mason - its a very good book and has chapters
devoted to this question. It is published by Academic Press and the ISBN is
0-12-477830-5.

I have heard that a new edition may be due later this year - maybe Bill
Mason can comment on that -

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-----Original Message-----
From: Confocal Microscopy List
[mailto:[log in to unmask]]On Behalf Of Doug Hubatsch
Sent: Wednesday, September 30, 1998 3:41 PM
To: [log in to unmask]
Subject: calcium dye question?


Hi All,

Anybody know what happens to the calcium and/or the dye after they have
bound together and been through a cycle of excitation and emission?  Does
the dye undergo a physical change (?) which results in the calcium being
released?   If so, is the dye 'weaker' during the next cycle?

Regards,

Doug