Dear Justin,

Try 7-aminoactinomycin D, which can be excited by 568 nm line of
Kr/Ar laser and emits red fluorescence (>610 nm).  This DNA dye just works fine in
fixed cells without needing any permeabilisation or digestion procedures,
just as DAPI in UV mode or hematoxylin stain in bright field.

Hope this helps.  Good luck.


> What nuclear stains are available for fixed cells that are excitable
> by either the 488 or 568 laser lines of a Krypton/Argon laser? Has
> anyone tried Sytox green from Molecular Probes on
> paraformaldehyde and  triton fixed cells?
>
> Thanks
>
> Justin
>

***************************************
Tetsuhiro Minamikawa, MBBS, PhD
Dept. Biochem & Mol Biol
Monash University
Wellington Road, Clayton, Victoria 3168
Australia
Ph: +61 3 9905 1430
Fax: +61 3 9905 4699
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