Acridine Orange will label vesicles.  It will give higher degerees of staining
with longer incubation times.  Unfortunately it is not terribly specific for
one type of vesicle over another.  It is taken up in living cells.


On Nov 5,  1:18pm, Mario Moronne wrote:
> Subject: organelle labels
> Dear Confocalists,
>
> We are conducting studies on the uptake of surface modified inorganic
> luminescent particles in living cells and wish to trace their movements. We
> would like opinions as to what the best organelle dyes are to use for
> colocalization studies including  lysosomes, ER, Golgi, mitochondria, etc.
> I realize that this is an exceedingly broad topic and those who choose to
> respond might consider responding to me directly. I have looked through the
> Molecular Probes product list but I have little direct experience with live
> cell imaging and would value your suggestions.
>
> As to a more specific question, I would also appreciate suggestions for
> vital stains. Calcein-AM seems to be a good candidate.
>
> Thanks to everyone,
>
>
> Mario M. Moronne, Ph.D.
> Life and Material Science Divisions M/S 6-2100
> Lawrence Berkeley National Laboratory
> 1 Cyclotron Rd.
> Berkeley, CA.
> 94720
>
> ph 510-486-4236
> FAX 510-486-5664
>
> [log in to unmask]
> [log in to unmask]
>-- End of excerpt from Mario Moronne



--
Jonathan T. Boyd
Fox Chase Cancer Center
7701 Burholme Ave.
Philadelphia, PA 19111
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