CONFOCALMICROSCOPY Archives

November 1998

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Subject:
Re: DNA stain for fixed plant cells
From:
Daniel B Szymanski <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 13 Nov 1998 18:27:10 -0600
Content-Type:
text/plain
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text/plain (45 lines) 
Two of the syto from molecular probes do fairly well.

Syto 13 and syto 19.  They can be purchased individually or as a sampler kit.

good luck,

dan

S
> We would like to visualize DNA in fixed Arabidopsis tissue using the 543 nm
> laser line and our LSM410.
>
> We have tried propidium iodide with little success!
>
> Could anybody suggest a dye (and staining procedure) that might work better?
>
> Thanks!
>
> Benedikt
>
> ------------------------------------------------------------------------------
> --
> Dr. Benedikt Kost
>
> Lab of Plant Molecular Biology
> RU box 162
> The Rockefeller University
> 1230 York Avenue
> New York, NY 10021-6399
> USA
>
> tel     +1 212 327 8045
> fax     +1 212 327 8327
> e-mail  [log in to unmask]


Dan Szymanski
University of Minnesota
Dept. of Genetics and Cell Biology
250 Biological Sciences Center
1445 Gortner Ave.
St. Paul, MN. 55108-1095
[log in to unmask]
fax (612)  625 5754

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