I'm studying the transport and localization of some proteins in the
cytoplasm of cultured cells. The cells are fixed in paraformaldehyde and
stained with a avidin-biotin-fluorescein system.
I need to do double staining for this protein and the Golgi, so I intend
to use the Bodipy-tr-ceramide probe, as suggested by Molecular Probes  .

I would like to know if someone has experience or worked with this
specific probe. Particularly I need to know if this probe may be used
with fixed cells, that is, after the cells are fixed.

I will be very grateful for any information

Thanks in advance

Francisco Javier Hernandez-Blazquez
IARC-WHO
Unit of Multistage Carcinogenesis
150 Cours Albert Thomas
Lyon - France

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