At 11:40 AM 7/14/1999 +0000, you wrote:
>Hi all,
>I´m trying to determine EXTRAcellular calcium concentration using
>magfura2. During the protocol I need to add vanadate, but this ion
>decreases the fluorescence of mag fura. Does anybody know if some
>other calcium dye is insensitive to vanadate? I was thinkin of fluo-3
>and chlorotetraciclin.
>Thanks
>
>Dr Pedro J Camello
>Dpt Physiology
>Fac Veterinary Sciences
>Univ of Extremadura
>PO Box 643
>10071 Caceres
>SPAIN
>
Yes vanadate should not be a problem if you use fluo 3 or Ca green (I used
it already). I guess vanadate affects UV signals.

good luck

michel
Michel Puceat, Ph.D
Centre de Recherches de Biochimie Macromoleculaire
CNRS UPR 1086
1919 Route de Mende
34293 MONTPELLIER, Cedex 5, France
Phone  lab:(33) (0)4-67-61-33-50 or 33-54 
Fax: (33) (0) 4-67-52-15-59 
http://www.crbm.cnrs-mop.fr/homegb.html
ftp://ftp.crbm.cnrs-mop.fr/users/gtpases/puceat