CONFOCALMICROSCOPY Archives

July 1999

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From:
Aryeh Weiss <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 15 Jul 1999 20:22:27 +0300
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I found that when imaging a uniform fluorescent block, the resulting image is
not uniform (even well beyond the acceptance criterion). Even after allignment
of the
mirrors, there can be a variation of as much as 50 grey level across the field
at zoom=1.

What is worse -- the variation across PMT3 do not track the variations across
PMT1 and PMT2. The variation across PMT1 and PMT2 track reasonably closely, but
not perfectly.
This means that it is not just nonuniform illumination.
It also means that comparing relative strengths between channels is difficult,
and in the case of PMT3, probably not possible at zoom=1.

For higher zooms, the results are of course better, but even at zoom=2 PMT3 is
not great.

Is there any experience out there -- particularly among MRC-1024 users -- that
may
be useful for me on this problem?

Thanks in advance
--aryeh
--
Aryeh Weiss                          | email: [log in to unmask]
Department of Electronics            | URL:   http://optics.jct.ac.il/~aryeh
Jerusalem College of Technology      | phone: 972-2-6751146
POB 16031                            | FAX:   972-2-6751275
Jerusalem, Israel                    | ham radio: 4X1PB/KA1PB

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