Dear Mark,

I suspect that you will use the DIC optics for looking at oocytes as
your adress refers to a department of obstetrics, please note that
changing to infinity objectives of NIKON will increase your working
distance and hence will allow you to go trough the whole cell of 100
mucron. This is again an argument in favor of the eclipse 300 microscope.

Patrick

On Wed, 7 Jul
1999, Dr. Mark W. Tengowski wrote:

> Greetings again,
>
> I am thinking about adding DIC optics to my current confocal system (Nikon
> Diaphot 200, BioRad 1024 scanhead, Kr/Ar laser, transmitted light
> detector).  Before getting quotes, I have a few questions to ask the list
> in hopes that someone has been successful in this type of endeavor.
>
> Is it worth upgrading the Nikon base, since objectives are mostly discontinued?
>
> While it seems important for one of my more animated user, is adding DIC
> optics really a cost-effective upgrade?  The TLD currently sees limited use.
>
> If anyone has DIC merged with confocal pictures, please send me a jpg or
> reference.  I can tweak the TLD images to look more like phase, but the
> detail of DIC is definitely lacking.
>
> Respectfully,
> -Mark
>
> Mark W. Tengowski, DVM, MS, PhD
> University of Wisconsin Medical School
> W. M. Keck Neural Imaging Laboratory
> Department of Obstetrics & Gynecology
>

Patrick Van Oostveldt
Lab. Biochemistry & Molecular Cytology
Coupure Links 653
B9000 GENT

tel: 32 (0)9 264 5969
fax: 32 (0)9 264 6219