>Date:         Wed, 27 Oct 1999 13:53:12 -0400
>Reply-To: Confocal Microscopy List <[log in to unmask]>
>From: Michael Roper <[log in to unmask]>
>Subject:      image analysis
>
>Using software (Simple 32, v. 2.0) that came with our CLSM, we are able
>to take intensity values for each pixel and place them in a
>spreadsheet.
>However, it does not seem possible to place these values back into an
>image.  The only way, that I have found, to graph these pixel values is
>by Excel which obviously does not give the best image quality.  Does
>anyone know of a program that can take intensity values for each pixel
>from a spreadsheet format and then return these values back to an
>image?
>Thanks.
>
>Mike
>
>Michael Roper
>University of Florida
>Department of Chemistry
>PO Box 117200
>Gainesville, FL 32611
>352-846-0838
>[log in to unmask]

In a Mac environment, Object-Image would be the way to go; if wintel
then Scion Image available via NIH-Image webpages will also happily do
this with Excel. Both are freely available and based on public domain
NIH-Image.

Object-Image
------------
by Norbert Vischer,  University of Amsterdam
e-mail:    [log in to unmask]
www:       http://simon.bio.uva.nl/object-image.html
ftp:       ftp://simon.bio.uva.nl/pub/

This program is based on Wayne Rasband's
NIH Image
---------
www:        http://rsb.info.nih.gov/nih-image
ftp:        ftp://codon.nih.gov/pub/nih-image/
mailing list: [log in to unmask]

Greg Joss,
School of Biological Sciences, Phone:(61)(2) 9850 8212 Fax: 9850 8174
Macquarie University,          Email [log in to unmask]
North Ryde, (Sydney,) NSW 2109, Australia