I can also attest to the value of DiO for staining in airway epithelia and
cultured cells.

Carla Ribeiro wrote:

> Francisco Hernandez wrote:
>
> Dear Members
>
> I need to immunostain the endoplasmic reticulum of culture cells in
> order to localize a protein (I'm intending to do double staining). Could
> someone suggest me a good antibody marker of ER in mammals cells?
>
> Thanks in advance
>
> Dear Francisco,
>
> For ER labeling I would suggest a calreticulin antibody from Affinity
> Bioreagents -- I have labeled the ER from NIH3T3 fibroblasts, HEK293
> cells, jurkat cells and human airway epithelia successfully. This
> antibody is also excellent for Western blots, giving a single clear band
> of ~ 60 kD. You can also use DIOC(6)3, a fluorescent
> dihexaoxacarbocyanine dye extensively characterized by Mark Terasaki and
> coworkers.
>
> Good luck with your ER labeling,
>
> Carla Ribeiro.
>
> Carla M. Pedrosa Ribeiro
> Cystic Fibrosis Pulmonary Research and Treatment Center
> 7013 Thurston-Bowles Bldg., CB#7248
> Chapel Hill, NC 27599
> Phone: (919)966-7045
> e-mail: [log in to unmask]

--
Dr. Anthony G. Moss
Associate Professor
Biological Sciences
131 Cary Hall
Auburn University
Auburn, AL  36849-5414

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email: [log in to unmask]
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