Peter-
        Thank you very much for this reference.  However, I looking for
a fluorescent stain and I believe these do not fluoresce.  Is that
correct?

Wendy

> ----------
> From:         Angell, Peter[SMTP:[log in to unmask]]
> Reply To:     Confocal Microscopy List
> Sent:         Tuesday, October 26, 1999 6:16 AM
> To:   [log in to unmask]
> Subject:      Re: Alginate stain
>
> Periodic acid-Schiff reagent will stain carbohydrates, especially
> polysaccharides.
>
> Alcian blue/yellow is used for anionically substituted
> polysaccharides.
>
> Methods have been described by A.H.L. Chamberlain, P. Angell and H.S.
> Campbell  "Staining procedures for characterising biofilms in
> corrosion
> investigations"  British Corrosion Journal, 1988, Vol. 23, No 3  pp.
> 197,198
>
> Both of these stains are non-UV. the paper gives a procedure used for
> removing and staining biofilms from copper pipes, but also gives the
> traditional references for the staining techniques.
>
> Hope this helps
>
> Peter
>
> > ----------
> > From:         Cochran, Wendy[SMTP:[log in to unmask]]
> > Reply To:     Confocal Microscopy List
> > Sent:         October 25, 1999 5:09 PM
> > To:   [log in to unmask]
> > Subject:      Alginate stain
> >
> > I am looking for a fluorescent (non-UV excited) stain for bacterial
> > alginate.  Any suggestions?
> >
> > Wendy
> >
>