CONFOCALMICROSCOPY Archives

December 1999

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Subject:
From:
Aryeh Weiss <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 9 Dec 1999 09:53:46 +0200
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"Reece.Jeffrey" wrote:
>
> Yes, "multitracking" is switching between different excitations and
> emissions (split to different PMTs) on a scanline basis.  You could for
> instance have three probes-- scan one on the forward scanline, probe two on
> the flyback, probe three on a redo of the same scanline, and blanked out on
> the flyback, then on to the next line.

Has anyboby checked the correspondence between pixel coordinates for the
forward and flyback trace. That is, if a point is imaged at (for example)
point x=100 (100th point out of 512) in the forward direction, will it occur at
x=412 (that is, 100 points from the end) in the reverse direction.

This should be checked because there may be some scan nonlinearity due to the
need for the galvos to come up to speed, and this nonlinearity can be different
(mirror image?) for the flyback.

--aryeh
--
Aryeh Weiss                          | email: [log in to unmask]
Department of Electronics            | URL:   http://optics.jct.ac.il/~aryeh
Jerusalem College of Technology      | phone: 972-2-6751146
POB 16031                            | FAX:   972-2-6751275
Jerusalem, Israel                    | ham radio: 4X1PB/KA1PB

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