CONFOCALMICROSCOPY Archives

December 1999

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Subject:
Re: Secondary Antibodies
From:
Martin Wessendorf <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 6 Dec 1999 11:38:48 -0600
Content-Type:
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Kathy Terry wrote:
>
> Good Morning Confocal Gurus!
>
>         I have a single (possible silly) question.  Can solutions of
> secondary antibodies (conjugated to fluorophores, used to label whole
> mounts of insect brains) be re-used?  Thanks!  Ciao!

Yes, as can solutions of primary antibodies.  In some cases, labeling
improves with time as non-specifically bound components are gradually
removed.

Good luck!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                        office: (612) 626 0145
Associate Professor                             lab:    (612) 624 2991
Dept. Neuroscience                      Preferred FAX:  (612) 624 8118
University of Minnesota                 Dept FAX:       (612) 626 5009
Minneapolis, MN  55455                e-mail: [log in to unmask]

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