At 04:55 PM 12/10/99 -0600, you wrote:
>Has anyone performed calcium imaging on a LSM 510 UV confocal?  What is a
>good positive control? We are using Fura-2 and our lines of excitation are
>351 and 364 (compared to 340 and 380). After creating conditions that would
>cause a calcium influx, no significant change in the ratio was observed. Is
>it possible that the cells are not behaving as they should? The cells that
>we are using are smooth muscle cells. Any comments are appreciated.

A more typical way to perform calcium imaging on a UV confocal is to use
Indo-1 instead of Fura-2.
 I doubt that you will get much of a ratio change using Fura with  351/364
nm. You could just set up some calcium standards to check .

John

Dr. John Cork,
Calcium Imaging Facility
Department of Cell Biology &  Anatomy,
LSUMC, 1901 Perdido St., New Orleans
LA 70112

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