CONFOCALMICROSCOPY Archives

July 2009

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Condense Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Sender:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 15 Jul 2009 15:37:01 -0600
Reply-To:
Confocal Microscopy List <[log in to unmask]>
Subject:
Re: VisEn Prosense 750 on multiphoton?
MIME-Version:
1.0
Content-Transfer-Encoding:
quoted-printable
In-Reply-To:
<[log in to unmask]>
Content-Type:
text/plain; charset=ISO-8859-1
From:
Craig Brideau <[log in to unmask]>
Parts/Attachments:
text/plain (27 lines) 
For multiphoton (in this case 2 photon) won't you need a very long
wavelength?  Probably >1200nm?  Ti:Saphs don't go beyond 1100nm so you
won't be able to use multiphoton to stimulate it unless you have a
Cr:Forsterite laser lying around or an OPA/OPO system strapped to your
Ti:Saph.  If you want to use single photon you can just tune your
Ti:Saph to 750 and you should be good to go.

Craig


On Wed, Jul 15, 2009 at 1:30 PM, Gastinger, Matthew (NIH/NIAID)
[C]<[log in to unmask]> wrote:
> We have someone who wants to use ProSense 750 (a protease activatable
> fluorescent in vivo imaging agent) from VisEN.  He wants to image in vivo
> using confocal microscopy.  The alternative is ProSense 680, but 750 is
> likely to produce less autofluorescence.  ProSense 680 will likely work with
> 633 laser or whitelight laser at 670nm.
>
>
>
> Has anyone imaged VisEN ProSense 750 using multiphoton confocal microscopy?
> If so, what are equipment specs generating images.
>
>
>
> Thanks.

ATOM RSS1 RSS2