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Hi everyone,
I was wondering if anyone has successfully used the Alexa Fluor 532 
secondary to view tyrosine hydroxylase in dopaminergic neurons. I'll be 
imaging it using a 543nm laser with a Nikon confocal microscope. Based on its 
excitation wavelength it seems to be an ideal match but I don't any 
experience with it. 

If not, can anyone comment on its anti-quenching properties? I'm currently 
using the Alexa Fluor 546 for the same purpose but it quenches almost 
unusably fast with every mounting media I've tried.

Thanks for the help,
Scott Wong