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Dear Martin,

Unfortunately, we do not have time to make a really quantitative
comparison but our day to day experience says that with a spinning disk
(irrespective whether EM-CCD or sCMOS) we have significantly better
results on thin, weak, dynamic samples (like visualizing microtubules in
yeast) then with a high-end confocal (Leica SP8 with HyD-s and 8kHZ
resonant scanner). With our sCMOS based system we can get a 4MP (so
relatively large FOV) image with 10-100 ms camera integration time for a
"standard" (of course this is ill defined) GFP labeled sample without
significant bleaching. This is (to my knowledge) impossible with any point
scanning system. 

Greetings    Gabor


On 4/22/15 11:10 AM, "Martin Offterdinger"
<[log in to unmask]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Hi
>There had been a discussion on that topic already 10 years ago...
>I was wondering if it is really still valid to say that a spinning disk
>microscope with an EM-CCD is always better for fast live imaging than a
>latest generation fast scanning LSM equipped with sensitive detectors- we
>have a Leica SP5 with RS and HyDs. 10 years ago people argued that with
>the
>PMTs you will loose a lot of light compared to the SD system. But now we
>do
>have much better detectors on the LSM such as GaSPs and HyDs!  Is it
>really
>still a big advantage to use a spinning disk system compared to a
>confocal.
>Does anyone know a systematic side by side comparison in terms of
>signal-to-noise, speed, bleaching properties...?
>
>In other words- is it still better to go for spinning disk, when you need
>a
>fast live cell imaging or can a fast & sensitive LSM truly compete?
>
>
>Best 
>Martin
>***********************
>Martin Offterdinger, PhD
>Medical University Innsbruck
>CCB
>Division of Neurobiochemistry
>Biooptics
>Innrain 80-82, 1st floor, room 01.370
>A-6020 Innsbruck
>Austria
>***********************
>phone number: +43-512-9003-70287
>http://www.i-med.ac.at/neurobiochemistry/neurobiochemistry/Biooptics/Main.
>html