THE PROBLEM:
We study living cells and have some problems with temperature
controlling. It seems that a variation of less than 0.1 degree C influences the
focal depth too much. The focal depth changes sometimes 0.5 um.
THE SET-UP:
1) 60x/1.4 Nikon (on a Biorad MRC 1000/1024)
2) an objective heater and its controller.
3) cells on a Glass Bottom Petri dish (MatTek)
4) a 'warm table' for the petri-dish and its controller
The heater controllers keep the temperature on 37 C with an accuracy
of less that 0.1 C so, that seems to be good enough.
THE QUESTION:
1) Has anyone similar problems?
2) Did anyone solve the problem?
Thank for your reply, Erik Manders
CRC Nuclear Structure and Function Group
Sir William Dunn School of Pathology
Oxford University
South Parks Road, Oxford OX1 3RE, UK
Tel: (+44/0) 1865 275529 (/275500)
Fax: (+44/0) 1865 275501
E-Mail: [log in to unmask]