Dear Ray, You could use a Sykes-Moore chamber. this is a very simple chamber available from Bellco glass Co. "HTTP://WWW.BELLCOGLASS.COM/sect2.htm" (No commercial affiliation with Bellco Glass) Stephen H. Cody, Colon Molecular and Cell Biology Laboratory, Ludwig Institute for Cancer Research, Post Office Royal Melbourne Hospital, Parkville, Victoria 3050, Australia. Tel: 61 3 9341 3155 Fax: 61 3 9341 3104 email: [log in to unmask] www.ludwig.edu.au/confocal > ---------- > From: ray hester[SMTP:[log in to unmask]] > Reply To: Confocal Microscopy List > Sent: Thursday, August 24, 2000 12:29 AM > To: [log in to unmask] > Subject: Upright scope with closed culture system and 100x objective? > > Hi, > > Is anyone using an upright fluorescence microscope to analyze viable cells > in a closed culture system with a 100x oil objective. i.e., the cells will > have to be upside down on the upper cover glass and the chamber filled > with > culture fluid? > > If so, did you have to make your culture holder or are there commercially > available systems that will accomplish this? The Bioptechs catalog shows > the FCS3 which would permit this but I'm not sure if it's in production > yet. > > Thanks for any help. > > Ray > > Raymond B. Hester, Ph.D. > Research Cytometry Laboratory > CSAB 357, Biotechnical Services > University of South Alabama > Mobile, AL 36688 > Voice: (334) 460-6029 > FAX: (334) 460-6073 > E-mail: [log in to unmask] >