Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Andy: I have looked at your web site and have been following some of the developments in Qdot technology in the literature. This looks very promising. Does your company plan to market 1) the dots themselves, since we would be interested in using them as markers of endocytosis, and 2) dots plus protein coupling technology, so we could label our own proteins and antibodies? David Dr. David S. Miller NIH/NIEHS 919 541 3235 FAX: 919 541 1898 -----Original Message----- From: Andy Watson [mailto:[log in to unmask]] Sent: Tuesday, November 19, 2002 7:20 PM To: [log in to unmask] Subject: Quantum dots now available Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal First, I apologize if this announcement is too commercial in nature - however several academic confocal microscopists recommended that I make a post to this listserver to tell you about a new type of fluorescence probe that we believe will revolutionize fluorescence microscopy. We have now released the first qdot bio-labeling product. These are built upon quantum dot nanocrystal technology - nanometer sized semiconductors that fluoresce very brightly. Details of the photostability, spectra, quantum yield, and staining protocols are available on our website <http://www.qdots.com/> www.qdots.com. In summary, we have observed complete photostability (without any anti-fade additives), and brightness often greater than an order-of-magnitude higher than conventional fluorescence dyes. These data were generated using epi-fluorescence microscopes and fluorescence plate readers. We have only limited data in confocal microscopy but the data we do have suggests that these benefits appear also to extend to confocal microscopy. This first quantum dot color (605nm emission) can be excited with a 532, 488, 405, or UV laser. Shorter wavelengths are better, but all of these wavelengths work well. This first color has quantum yield around 50%, and has a very narrow emission (<27nm FWHM) with no red-tailing. Additional colors will be launched in January and further conjugation methods will be available too throughout 2003. The first kit retails for $600, and contains the Qdot 605 streptavidin conjugate, sufficient for at least 100 staining experiments, along with a specially formulated incubation and dilution buffer. Andy Watson Quantum Dot Corporation 510 887 8775 www.qdots.com <http://www.qdots.com/> [log in to unmask] <mailto:[log in to unmask]>