***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 Post images on http://www.imgur.com and include the link in your posting. ***** Dear All, We have a W1 spinning disk with dual cameras. We recently had them aligned, and upon close inspection, we see that the GFP channel image is about 1 to 2 pixels larger than the RFP image in every corner (we used a 60x objective, and the cameras are primeBSI express with 6.4um pixels, so the image is off by 100-200nm in every corner). you can download the two images as a composite here if you are curious: https://drive.google.com/file/d/1TWIxwiZF6uf3FspyA96AX6r092PB_102/view?usp=sharing What would be the best way to solve this issue? Shouldn't the standard camera mounts enable us to correct this? Thanks for your help and comments, Best wishes, Emmanuel