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Dear Confocal Microscopy List
Years ago I got a very useful protocol from somebody on the List for making live imaging dishes. Basically, one burred holes in a Petri dish and glued a suitable coverslip beneath using World Precision Instruments, #SYLG184. We continue to use Slyfard (R) 184, but we can't keep the cells alive. It seems like the glue is somehow toxic to the cells - yet we had great success in the past.

Any advice will be greatly appreciated.
Best wishes
Julia