***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 Post images on http://www.imgur.com and include the link in your posting. ***** Dear Confocal Microscopy List Years ago I got a very useful protocol from somebody on the List for making live imaging dishes. Basically, one burred holes in a Petri dish and glued a suitable coverslip beneath using World Precision Instruments, #SYLG184. We continue to use Slyfard (R) 184, but we can't keep the cells alive. It seems like the glue is somehow toxic to the cells - yet we had great success in the past. Any advice will be greatly appreciated. Best wishes Julia