***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 Post images on http://www.imgur.com and include the link in your posting. ***** Michael, I hope I'm wrong about this, but it reminds me of images of mycoplasma-infected cells. Those cells also don't look very healthy. Could they be infected? Jason Jason A. Kilgore Supervisor, Cell Analysis Tech Support Life Sciences Solutions Thermo Fisher Scientific -----Original Message----- From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Model, Michael Sent: Thursday, April 11, 2024 4:38 PM To: [log in to unmask] Subject: Re: EXT: Re: vesicles CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to [log in to unmask] ***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://urldefense.com/v3/__https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1__;!!HLrAl2XzZ3iCLg!B9GBDpQnxu1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_uknNFqmJrTAD$ Post images on https://urldefense.com/v3/__http://www.imgur.com__;!!HLrAl2XzZ3iCLg!B9GBDpQnxu1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_uknNFuWcJGpZ$ and include the link in your posting. ***** Thank you, Tim, But I don't think this is it. I placed one of the images here: https://urldefense.com/v3/__https://www.dropbox.com/scl/fo/3p8yfmm5rqoo1rqj36bkd/AJOTl2uNKOh6DSXztwO2HWI?rlkey=h771q3h1abhtssv327dng2yre&dl=0__;!!HLrAl2XzZ3iCLg!B9GBDpQnxu1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_uknNFgMjkrAq$ -----Original Message----- From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Tim F Sent: Thursday, April 11, 2024 5:24 PM To: [log in to unmask] Subject: EXT: Re: vesicles ***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://urldefense.com/v3/__https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1__;!!HLrAl2XzZ3iCLg!B9GBDpQnxu1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_uknNFqmJrTAD$ Post images on https://urldefense.com/v3/__http://www.imgur.com/__;!!HLrAl2XzZ3iCLg!B9GBDpQnxu1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_uknNFohxnDzh$ and include the link in your posting. ***** Hi Michael, just to confirm, if your experience is similar to the video below, do you mean the dark spots? Thanks to Nikon for the vid! https://urldefense.com/v3/__https://youtu.be/Rb-Q3wLsbIk?si=f8GSrHrkTEYmZg1t__;!!HLrAl2XzZ3iCLg!B9GBDpQnxu1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_uknNFk1AOE-7$ I remember thinking those are protein mis-folding aggregates. I don't think you would see endosomes with that kind of contrast, and vesicles are too small at 60nm to see with transmitted light. Best, T Timothy Feinstein, Ph.D. On Thu, Apr 11, 2024 at 3:53 PM Model, Michael <[log in to unmask]> wrote: > ***** > To join or leave the confocal microscopy listserv or to change your > email address, go to: > https://urldefense.com/v3/__https://list/__;!!HLrAl2XzZ3iCLg!B9GBDpQnx > u1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_ukn > NFn86y8bg$ > s.umn.edu%2Fcgi-bin%2Fwa%3FSUBED1%3Dconfocalmicroscopy%26A%3D1&data=05 > %7C02%7Cmmodel%40KENT.EDU%7C560e6be681534059186e08dc5a6e4300%7Ce5a06f4 > a1ec44d018f73e7dd15f26134%7C1%7C0%7C638484676935174256%7CUnknown%7CTWF > pbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6M > n0%3D%7C60000%7C%7C%7C&sdata=6AJ4ERdzHUxtlMrfZG2aSTCg8fzFtpz9J3jiAK37H > i0%3D&reserved=0 Post images on > https://urldefense.com/v3/__http://www.imgur.com/__;!!HLrAl2XzZ3iCLg!B9GBDpQnxu1Zw-80F71B9xaYdV0k6k1-N7cd7igG9A0s7AAXrs4NrHC1Yq7PitL8kMdg_Gd8sKH_uknNFohxnDzh$ and include the link in your posting. > ***** > > Dear List, > > If you look at cultured cells, such as HeLa, under bright field, you > clearly see many vesicles about 1 um. Does anyone know what they are? > (They are not acidic). The stupidest thing is that I once found a > paper that investigates them, but now I cannot find that paper! > > Mike > CAUTION: EXTERNAL SENDER Do not click any links, open any attachments, or REPLY to the message unless you trust the sender and know the content is safe.