***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 Post images on http://www.imgur.com and include the link in your posting. ***** Hi Jeremy. Comet artifact is when HyD becomes excited for much longer time the usual nanosecond response. Then you can see a comet like signal with tail in direction of scanning. On this image is not the best example https://photos.app.goo.gl/sv9AYVjN22NYBzte7 But you can see a few. A nice example to have a look on noise without signal, but I did not save it. To fix it I was told a few methods. - To wait. Did not help in my case. - To take detector out and light it with a flash light. I have not tried it. - To saturate it with a signal that it just start to beep. I used convalaria slide and ~15-20 sec of beeps. In my case the comets disappeared. As for reason why they come, I do not know. It could be, as mentioned here, that someone was severely overloading it, we got a few new users last week and I can't babysit them all the time. Best. Petro. On Mon, 15 Apr 2024, 11:10 Jeremy Adler, <[log in to unmask]> wrote: > ***** > To join or leave the confocal microscopy listserv or to change your email > address, go to: > https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Petro, > > Could you explain what the Hyd comet artefact is/was. > > Is the fix, software is so which version. > > Best > > > =============================================== > B i o V i s P l a t f o r m of Uppsala University > Light & EM microscopy / FlowCytometry & Cell Sorting / > Image Analysis > =============================================== > Jeremy Adler PhD - Senior research engineer > Light, Confocal Microscopy, Image Analysis > E-mail: [log in to unmask] > 0739 188170 > > Dag Hammarskjölds v 20 > 751 85 UPPSALA, SWEDEN > http://biovis.uu.se/ > =============================================== > > > > -----Original Message----- > From: Confocal Microscopy List <[log in to unmask]> On > Behalf Of Khoroshyy Petro > Sent: Monday, April 15, 2024 10:53 AM > To: [log in to unmask] > Subject: Re: HyD comet artifact? > > ***** > To join or leave the confocal microscopy listserv or to change your email > address, go to: > https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi. > Sorry. > It looks like I posted to this list instead of our facility users mailing > list. > My apologies. > Petro. > > > On Mon, 15 Apr 2024, 10:11 Khoroshyy Petro, <[log in to unmask]> wrote: > > > Hi everybody. > > The comet artifact is fixed. > > If you have it anytime I future. > > Please let me know. > > Best regards. > > Petro. > > > > On Sun, 14 Apr 2024, 09:20 Khoroshyy Petro, <[log in to unmask]> wrote: > > > >> Hi everybody. > >> Could you educate me about the comet artifact of HyD and why it happens? > >> Is there anything I can do to prevent this from happening? > >> What is the latest information on it? > >> Thanks. > >> Petro. > >> > >> -- > >> ______________________________ > >> Petro Khoroshyy > >> > > > > VARNING: Klicka inte på länkar och öppna inte bilagor om du inte känner > igen avsändaren och vet att innehållet är säkert. > CAUTION: Do not click on links or open attachments unless you recognise > the sender and know the content is safe. > > > > > > > > > När du har kontakt med oss på Uppsala universitet med e-post så innebär > det att vi behandlar dina personuppgifter. För att läsa mer om hur vi gör > det kan du läsa här: http://www.uu.se/om-uu/dataskydd-personuppgifter/ > > E-mailing Uppsala University means that we will process your personal > data. For more information on how this is performed, please read here: > http://www.uu.se/en/about-uu/data-protection-policy >