***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 Post images on http://www.imgur.com and include the link in your posting. ***** Hi Michael, just to confirm, if your experience is similar to the video below, do you mean the dark spots? Thanks to Nikon for the vid! https://youtu.be/Rb-Q3wLsbIk?si=f8GSrHrkTEYmZg1t I remember thinking those are protein mis-folding aggregates. I don't think you would see endosomes with that kind of contrast, and vesicles are too small at 60nm to see with transmitted light. Best, T Timothy Feinstein, Ph.D. On Thu, Apr 11, 2024 at 3:53 PM Model, Michael <[log in to unmask]> wrote: > ***** > To join or leave the confocal microscopy listserv or to change your email > address, go to: > https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear List, > > If you look at cultured cells, such as HeLa, under bright field, you > clearly see many vesicles about 1 um. Does anyone know what they are? (They > are not acidic). The stupidest thing is that I once found a paper that > investigates them, but now I cannot find that paper! > > Mike >