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Hi Michael, just to confirm, if your experience is similar to the video
below, do you mean the dark spots? Thanks to Nikon for the vid!

https://youtu.be/Rb-Q3wLsbIk?si=f8GSrHrkTEYmZg1t

I remember thinking those are protein mis-folding aggregates. I don't think
you would see endosomes with that kind of contrast, and vesicles are too
small at 60nm to see with transmitted light.

Best,


T

Timothy Feinstein, Ph.D.

On Thu, Apr 11, 2024 at 3:53 PM Model, Michael <[log in to unmask]> wrote:

> *****
> To join or leave the confocal microscopy listserv or to change your email
> address, go to:
> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear List,
>
> If you look at cultured cells, such as HeLa, under bright field, you
> clearly see many vesicles about 1 um. Does anyone know what they are? (They
> are not acidic). The stupidest thing is that I once found a paper that
> investigates them, but now I cannot find that paper!
>
> Mike
>