Lance Ladic writes: >P.S. As a followup question, if VoxelView will not allow me to obtain a more >accurate visual representation of the scale of my reconstructions in the >Z-plane, is there any other volume rendering software that will? Can't comment on VoxelView, but I have solved this very problem with AVS. It is a simple matter of adjusting the relative scale of the axies aplied to the ray tracer, using a 4x4 Euler transform. One can apply a floating point coefficient to the transform elements and come out with a ...what is the word...correctly proportioned image. For example where we use our 100x objective and have 512x512 images with x-y pixel sizes of ~0.1micron square, we use a z-axis step size of 0.5micron. To correctly render the image, we would simply multiply the axies by 1,1, and 5 in x,y, and z, respectively. (Actually though, this is also a good place to partially correct for focal anomalies introduced by mismatch in refractive indicies of immersion oil and mounting media, wherein z-axis stage movement does not equal focal point movement. The result being that your .5 micron stage movement turns out to be somewhat less in the collected image.) I was just last week trying to describe the nature of the problem, and I find it interesting to see the way it is put. There must be some word to describe the relation of correct dimensions. Something like "ortho-dimentional" or "iso-lateral", anyone have a good word for it? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Andy Jacobson <[log in to unmask]> <[log in to unmask]> Dept. Pharmacology / Div. Nuclear Medicine and Biophysics CHS B2-086 UCLA School of Medicine Phone:310-825-8584 Los Angeles, CA 90024-6948 Fax: 310-825-4517 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~