>The tiff file format is a 2dimensional image format. It does not usually conta >in several slices. This is usually done in several tiff files. > >Andrew Vaillant >University of Ottawa Centre for Confocal Microscopy >Ottawa, Canada Well, I know that NIH-Image, for one, is a program which allows you to save multiple-slice data sets in the TIFF format. These files are similar to Bio-Rad .PIC z-series files in that you can extract the individual files from these "stacks". You choose either the TIFF or PICS format (PICS is a multiple PICT format) when saving a stack. -- Charles Thomas USPA Licence A-19548 Integrated Microscopy Resource Univ. of Wisconsin-Madison 1675 Observatory Dr. Madison, WI 53706 608-263-6288 [log in to unmask] "I'd rather be skydiving..."