Hello, In regards to your second point, Molecular Probes, Inc. does a great job keeping track of most of their fluorescent indicators and probes. I'd simply like a short list of references where the Zeiss LSM410 has been used in published papers in the area of biology. I believe this would benefit your users and help to sell your microscope to prospective buyers. Wilfred Denetclaw Jr., Ph.D. On Fri, 27 Jan 1995, Zbigniew Iwinski wrote: > Greetings from Carl Zeiss Inc. > > In response to recent discussions and questions: > > 1.) "Vendor updates": > Carl Zeiss has always made software > updates available at no charge to the user. There has > been only one major upgrade to our LSM-310/410 > software. An announcement > was sent to our LSM-310/410 users asking > if they would reply in writing to receive > the update. > This helps us confirm that we have the proper > contact person on our mailing list (often a more > difficult problem than some might think). > > Occasionally minor revisions which are > essentially > "beta" versions of the software are available. > Since these may have unknown bugs caused by the > modifications > we do not do a general distribution > > A new release of the software will be available soon and > we will follow the same procedure for its > distribution. > > 2.) Re: References on published material with the Zeiss Confocal > > It is impossible for vendors with a large > installed customer base to maintain a current and > exhaustive list of publications. > > We do know the applications areas being > addressed by our users and can provide information > regarding people to contact for discussions > relating to details of use of our systems for > those applications. > > > Two of our users who manage centreal facilities are: > Dr. > > JoAnne Whallon at Michigan State University [and > is always willing to discuss confocal > applications] (517-355-0271). Dr. Matt Schibler > at LaJolla Cancer Research Foundation has also had > pretty broad experience (619-455-6480). > > A few recent references: > Sloane, B. F, et al, 1994, J. Cell Sci. 107: > 373-384 > > Durfee et al 1994, J. Cell Biol Nov '94 (also a > cover picture on that issue) > > Nishiyama and Stallcup, 1993, Mol. Biol. of the > Cell, 4: 1097-1108 (NG-2 protein) > > Krajewski et al, 1993, Canc. Res., 53: 4701-4714 > (BCL2 Oncoproteins) > > To add to our current list we would certainly > welcome updates > from our users (or friends). > 3.) On Tue, 17 Jan 1995, Samuel A. Tesfai wrote: > > >> variable 'save to disk' frequency? > > > Good question. I know some representetives from zeiss are subscribers > > and perhaps they are willing to take up this question. However, it is > > my understanding that you have to choose either "host memory" and then > > to disk, or directly to disk. Obviously the first would be faster. > > Your understanding and explaination are correct. > One has a choice during data acquisition to store images in the image > memory (frame grabber memory-the fastest), computer memory or > directly to the disk (the slowest). In the first two cases to store > images permanently one has to save them to the disk afterwards. The > speed depends on the image size (which is selectable) and say for images > of 512x64 one can acquire 15 frames per second in the image memory. > > Regards, > > Buddy Bossmann Tom Connelly Zbigniew Iwinski > Product Manager National Sales Manager Systems Engineer > Laser Scan Microscopes Microscope Systems Micro Systems > Carl Zeiss Inc. > > Please contact us by > phone at:. 914-681-7737 415-428-1513 914-681-7739 > fax at: 914-681-7846 (same) > > e-mail at: "[log in to unmask]" >