Mikael Gustafsson wrote: >I4m setting up an endothelial capillary culture system for microscopy >according to Cooke 1993. Does any one know where to find flat glass >capillaries with an optical quality suitable for high res microscopy ? Any >suggestions are welcome. Does any one have experience of such culture systems ? Dear Mikael, Ken Spring and I used the Vitro Dynamics (20 - 100 micron) pathlength rectangular capillaries for optical sectioning/deblurring calibrations with BCECF standards. This is reported in our paper in American Journal of Physiology 266 (Cell Physiology 35) C73-C80 (1994). Hope this is useful. Peter Harris ---------------------------------------------------------------------------- ------ Department of Physiology The University of Melbourne Parkville Victoria 3052 Australia [log in to unmask]