Can someone out there can help me get rid of, or at least reduce the autofluorescence from blood vessels in our sections. We do all of our imaging on brain slices (10-100 microns) fixed in 4% Paraform./PBS. I've tried various blocking buffers and routinely include glycine to help quench aldehydes to no avail. Any suggestions?? Thanks, Judy Drazba Judy Drazba, Ph.D. ([log in to unmask]) (Director, Confocal Microscopy Facility) Department of Neurosciences, NC-3 The Cleveland Clinic Foundation 9500 Euclid Avenue Cleveland, OH 44195-5001 Office (216)445-3760 Lab (216)444-8712 FAX (216)444-7927