Dear confocalists Like many other confocal users I want to study processes in living cells. To do this we use a Zeiss 410 confocal microscope setup, however there are no facilities to keep our cells at 37 degrees celsius. We can buy from Zeiss a heating plate, but this is a very expensive solution. I wonder what methods are used by others to keep cells alive. One of the problems we expect by using a heating plate is the heat drain from the lens just underneath our preparation. How do you measure the temperature ?. We are using cell preparations cultured on glass. All reactions are welcomed. ======================================================================== Gert van Cappellen, [log in to unmask] Erasmus University, Endocr.& Reprod., Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands Fax: +31 10 4366832