Symposium on: "Integrated Microscopy" September 29 to October 1, 1995 Organized by: Integrated Microscopy Resource (IMR) a Biomedical Research Resource at: The Wisconsin Center 702 Langdon Street Madison, WI 53706 PROGRAM FRIDAY (EVENING), SEPTEMBER 29, 1995 6:00 - 7:55 OPENING RECEPTION 8:00 - 8:45 Ken Jacobson, Univ. N. Carolina-Chapel Hill "Imaging the Traction Forces Exerted by Locomoting Fish Keratocytes" 8:50 - 9:35 John Sedat, Univ. California-San Francisco "Multi-dimensional Optical Microscopy and EM Tomography: New Approaches and Results" 9:40 - 10:25 Edward Salmon, Univ. N. Carolina-Chapel Hill "Multi-mode Light Microscopy of Microtubule Assembly Dynamics and Motor Proteins in Mitosis and Related Movements" SATURDAY, SEPTEMBER 30, 1995 8:30 - 9:15 D. Lansing Taylor, Carnegie-Mellon University "Molecular Dynamics of Force Generation during Cytokinesis" 9:20 - 10:05 Jeff Lichtman, Washington University "Imaging Synaptic Competition in Living Animals" 10:10 - 10:40 COFFEE BREAK 10:45 - 11:30 John White, Univ. Wisconsin-Madison "Evaluation of Multiple-Photon Excitation Fluorescence Imaging for In Vivo Studies" 11:35 - 12:20 Steven Smith, Stanford University "Dynamics of Hippocampal Dendrite Growth and Synaptogenesis" 12:25 - 1:25 LUNCH (on your own) 1:30 - 2:15 Gary Borisy, Univ. Wisconsin-Madison "Using Correlative Microscopy to Study the Dynamics of the Cytoskeleton" 2:20 - 3:05 Ralph Albrecht, Univ. Wisconsin-Madison "Using Correlative Microscopy to Follow Cell Surface Receptor Ligand Complexes and Associated Subjacent Intracellular Events" 3:10 - 3:55 Paul Bridgman, Washington University-St. Louis "Multiple Microscopic Techniques Applied to Growth Cone Intrapodia Fixed During Live Observation" 5:00 - 6:00 EXHIBITOR'S SOCIAL 6:00 - 8:00 BUFFET DINNER SUNDAY (MORINING), OCTOBER 1, 1995 9:00 - 9:45 Kent McDonald, Univ. California-Berkley "Cryotechniques: New Hope for the Ultrastructurally Challenged 9:50 - 10:35 Stanley Erlandsen, Univ. Minnesota "Leukocyte Rolling and Transendothelial Migration: Importance and Distribution of Cell Adhesion Molecules" 10:40 - 11:10 COFFEE BREAK 11:15 - 12:00 Hans Ris, Univ. Wisconsin-Madison "High-resolution FESEM Imaging of Internal Cell Structures after Epon Extraction from Sections: A New Approach to Correlative Ultrastructural and Immunocytochemical Studies" 12:05 - 12:50 Eric Henderson, Iowa State University "Biological AFM and Molecular Force Detection" FEES: General Registration $100.00 (On Site: $130.00) (Includes: Opening Reception, Social and Buffet Dinner, Coffee Breaks and Materials) Local Registration $ 80.00 (On Site: $110.00) (Includes: Opening Reception, Social, Coffee Breaks and Materials) FOR ADDITIONAL INFORMATION (including abstracts) CONSULT OUR WEB SITE http://www.bocklabs.wisc.edu/imr/imr.html TO RECEIVE A BROCHURE AND REGISTRATION FORM SEND COMPLETE MAILING INFORMATION TO: IMR Univ. Wisconsin-Madison 1675 Observatory Drive Madison, WI 53706 OR EMAIL: [log in to unmask] #*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#* Presentations will focus on biological problems for which a combination of microscopies [i.e. integrated microscopy] has been used. The speakers will demonstrate by example the power, potential and limitations of various microscopical techniques. The techniques which will be discussed include: DIC, Confocal, 2-Photon Excitation Imaging, SEM, TEM, Cryo-specimen Preparation, and AFM. #*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#* ******************************************************************************** Following the symposium, the IMR will be conducting a 2-day workshop (Oct 2 and 3). We will be presenting lectures and provide "hands-on" experience for the following techniques: * 2-photon excitation imaging * 4D DIC imaging * Cryo-SEM * High pressure freezing * Reversible embeddment for SEM and TEM Workshop attendence will be limited to 25 participants. A letter of application is required. Once accepted a fee of $150.00 will be due. ********************************************************************************