>We have been debating the pros and cons of an inverted against an >upright microscope for confocal applications. > >Are there any experiments that can be done ONLY using an upright ? > In brain slices we have been unable to do any experiments involving impaling cells from above for intracellular recording etc while observing from below. An inverted microscope is also not the best configuration for any slice experiments as the cells on the bottom of the slice have the least access to medium, oxygen, drugs etc. JOHN ____________________________________________ John Cork Calcium Imaging Facility, LSUMC, New Orleans. ----------------------------