Dear confocal people, I am now studying the tissue-specific distribution of a putative transmembrane receptor in Arabidopsis by fluorescence microscopy. According to the protocol I got for localization of a nuclear protein in corn, I have to treat thin sections with proteinase K which may have a detrimental effect to the protein I want to study. Does anyone have an idea about the rationale for apllying proteinase K? Or references discuss about it? Thanks in advance. Shinhan Shiu Dept of Botany, Univ of Wisc-Madison [log in to unmask]