Hi everybody. I want to look at fibres both in the native state and following contact with water. Since I need to examine the same portion of the fibre to determine the extent (percentage) of swelling, changes, deformation, etc, it becomes important not to change the position of the fibre. We have an inverted scope and need to look at the fibres using the 63x oil objective. I am toying with the idea of sealing our coverslips with silastic or perphaps vasoline. Hopefully this will prevent the coverslips falling off or moving when in contact with the immersion oil. Also, it will permit us to introduce water into the system (via a needle). Does anyone have any other comments or suggestions to make? Am I approaching my problem from the right direction? Does anybody else routinely study fibres in air and water environments? PLEASE HELP..... Thanks, Felicity Lawrence Analytical Electron Microscopy Facility Queensland University of Technology Brisbane, Australia Ph : +61 7 3864 2557 Fax : +61 7 3864 5100 Email: [log in to unmask]