Dear colleagues, We have been using confocal microscopy to investigate receptor cycling and co-localisation of receptors in endosomal and/or lysosomal compartments in T cell populations. However we are toying with the idea of sub cellular fractionation using Percoll , and further analysis of same compartments by gel electrophoresis etc. Specifically we would like to separate endosomes ( early , late ) from lysosomes . We would certainly appreciate correspondence from any others who have experience in sub cellular fractionation, on its own or as a compliment to confocal microscopy. Thank you [log in to unmask] Dept. of Biochemistry, University College, Galway, Ireland Tel: +353-91-524411 ext 2431 Fax:+353-91-525700