Hello everybody, I wonder if anyone has a program that would convert raw readings from a spectrofluorimeter (Indo /Fura) into Ca concentrations. Raw readings are F max, F min, and experimental F readings off your (precious) sample. Thank you for your time and advice. cheers michal Dr. Michal Opas Department of Anatomy & Cell Biology University of Toronto Medical Sciences Building Toronto, Ontario, M5S 1A8 Canada Please note new office phone #: office: (416) 971-2140 lab: (416) 978-8947 fax: (416) 978-3954 e-mail: [log in to unmask]