Form: Announcement Announcement: I'd appreciate getting any help regarding the use of scanning laser confocal microscopy for measurements in blood: I am using a confocal scanning laser microscopy system with which I intend to measure platelet aggregation in human blood in an in vitro flow loop (approx. 100ml of whole blood), using FITC for flourescence (conjugated with secondary antibody that binds to a primary antibody that binds to the platelet membrane). My question follows: do I have to label all the platelets, or is it enough to label only a small percentage of the platelets (e.g., 1% in PRP form?). The reason I am asking is because the conjugated FITC and the primary antibody are sold in very small amounts (1 ml bottles), and are fairly expensive. Your help would be greatly appreciated. Dr. Danny Bluestein Mechanical Engineering Department Florida International University